ABSTRACT
The rubber tree (Hevea brasiliensis) is native to the Amazon region, and it is widely exploited due to natural rubber produced from latex. There are many clonal varieties, without certification tests. In order to determine a genetic certification, 15 clones were genotyped to identify their genetic pattern. Ten microsatellites were used to determine a subset of alleles exclusive for each genetic profile. The genetic estimates obtained were: number of alleles per locus (N), expected (HE) and observed (HO) heterozygosity, Polymorphic Information Content (PIC) and Discriminatory Power (DP). The number of alleles (N) ranged from five to 14, with an average of 9.2. The HE mean (0.80) was higher than HO (0.60), indicating a selection for homozygotes. The locus informativeness was verified with PIC (0.77) and DP (0.90) means showing high polymorphism. The dendrogram represented the formation of three groups related to geographical origin. Clone MDF 180 presented the highest genetic divergence. Two genic pools represented the genetic composition of genotypes. Based on allelic profiles, a set of two microsatellites (A2365 and A2368) was able to distinguish all examined clones. The genetic certification using microsatellite fingerprinting proved to be an alternative to morphological traits.
Subject(s)
Genetic Variation , Hevea , Genetic Structures , Genetic ProfileABSTRACT
Portulaca oleracea has the functions of clearing heat and removing toxin, cooling blood to stop bleeding and treating dysentery. It is a medicine and food homologous drug with no toxic effect, which is common to people. As a drug, it has high safety. P. oleracea has a variety of active ingredients and pharmacological effects. In order to fully exploit the purslane and accelerate the modernization of purslane research, this paper summarizes the research progress of purslane. On this basis, the correlation analysis of “composition-activity-CMM efficacy-disease” was carried out, in order to provide ideas for the modern research of P. oleracea.
ABSTRACT
Characterization of the polysaccharides and monosaccharides of Bupleurum chinense was undertaken to identify differences in the Bupleurum chinense's sugar profiles, so as to provide a basis for the identification of different varieties. High performance liquid chromatography (HPLC) was used to generate chromatograms of the total polysaccharides of Bupleurum using an Evaporation Light Detector (ELSD), and a monosaccharide chromatogram was generated using a UV-detector (UV) following polysaccharide derivatization. The data were analyzed using SIMCA software and SPSS software to distinguish different varieties of Bupleurum. The results show that the yield of polysaccharides from Bupleurum falcatum is the highest, while the yield of polysaccharides from Bupleurum chinense is the lowest. The polysaccharide spectrum shows that the molecular weights of the polysaccharides in different Bupleurum differ, and their percentages of the total peak area are also different. The four Bupleurum polysaccharides are composed of mannose, glucuronic acid, rhamnose, galacturonic acid, glucose, galactose, and arabinose, but differ in length. The ratio of glucose to arabinose in Bupleurum chinense, Bupleurum scorzonerifolium, Bupleurum falcatum and Bupleurum marginatum var. stenophyllum is: 3.0-4.0, 5.5-7.0, 12.0-17.0, 9.0-12.0. In this study, a sugar profile technique was developed to provide a new method for the identification of different varieties of Bupleurum.
ABSTRACT
As a commonly used traditional Chinese medicine,Stellaria dichotoma var. lanceolata has a medicinal history of several hundred years, and been included in the Pharmacopoeia of the People's Republic of China. With a sweet fact and mild war nature, it enters liver and stomach meridians. With effects in clearing deficient heat and eliminating fever in infantile malnu, it has been used to treat such diseases as Yin deficiency fever, consumptive fever due to Yin deficiency and infantile chancre fever, and taken as the raw materials of Wuji Baifeng Wan and other traditional Chinese medicine preparations. In recent years,there have been increasing demands for S. dichotoma var. lanceolata from people. However, its wild resources have been over-excavated for a long time,leading to a serious shortage of wild strains. Furthermore, the quality of artificial S. dichotoma var. lanceolata medicine is far different from that of wild cultivars. Meanwhile,pesticide residues and excessive metal standards due to the irrational use of pesticides and chemical fertilizers in the production process, as well as the large number of counterfeits in the market seriously impact the quality,efficacy and drug safety of S. dichotoma var. lanceolata medicine. Therefore, the non-polluted production technology of S. dichotoma var. lanceolata is of great significance. The non-polluted production technology would be an effective mode for promoting the sound development of S. dichotoma var. lanceolata industry in the future and the key to solve the issues. This article summarizes the environment of suitable production area,plantation method, comprehensive soil improvement, field management and rational fertilization technology. It also proposes that the prevention and control of pollution-free safflower pests and diseases should follow the principle of giving priority to comprehensive prevention. The pollution-free and technical regulation system of S.dichotoma var. lanceolata cultivars is built to produce excellent,high-quality and non-polluted production with low content of pesticide residues and heavy metals,and promote the healthy and sustainable development of the global S.dichotoma var. lanceolata industry.
ABSTRACT
This study was aimed to establish the method of identifying bupleurum cultivated germplasm using simple sequence repeat (SSR) molecular markers and to initially establish dataset of characteristic SSR bands to the bred cultivars or strains. From the bupleurum SSR primer pairs which were designed in previous work, 50 primer pairs were selected. Two bred strains and 4 other bupleurum cultivated germplasms were used as test materials. Primers pairs were screened with effective PCR amplification and high polymorphism. Meanwhile, conditions for PCR amplification and electrophoresis were optimized. Then, obtained SSR bands were analyzed and a clustering tree on the basis of genetic distance was constructed. The results showed that 9 SSR primer pairs can be used for identification. The suitable assay conditions were established and characteristic SSR bands were obtained for tested materials. The tested samples can be divided into 4 categories in the genetic similarity coefficient of 0.73. TheB. scorzonerifolium cultivated inHeilongjiang andChuanhongchaiNo. 1 strains were clustered as one category. ChuanbeichaiNo. 1 strain andZhongchai No. 1 cultivar clustered as another category. Cultivated germplasms fromSichuan Fengshunand Rongxian clustered as a unique category. It was concluded that the primer pairs and assay method established in the present study can be used as reference in identification of bupleurum cultivars or cultivated germplasms.